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rabbit anti mouse tollip  (Boster Bio)


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    Boster Bio rabbit anti mouse tollip
    Figure 1. Effect of XBJ on the mRNA expression of <t>Tollip,</t> IRAK1, TLR4, NF-κB65 and TRAF6 in lung tissue. Groups of mice were challenged with CLP and treated with XBJ 24 h later. The expression of Tollip, IRAK1, TLR4, NF-κB65 and TRAF6 in lung tissue was determined by RT-PCR. Representative RT-PCR shows the level of Tollip, IRAK1, TLR4, NF-κB65, and TRAF6 expression in the four rat groups. M, marker; A, normal control group; B, sham operation group; C, control group; D, treatment group.
    Rabbit Anti Mouse Tollip, supplied by Boster Bio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+mouse+tollip/Anti-Toll-interacting+protein+TOLLIP+Antibody/pm25269519-60-0-15
    Average 90 stars, based on 1 article reviews
    rabbit anti mouse tollip - by Bioz Stars, 2026-10
    90/100 stars

    Images

    1) Product Images from "Xuebijing exerts protective effects on lung permeability leakage and lung injury by upregulating Toll-interacting protein expression in rats with sepsis."

    Article Title: Xuebijing exerts protective effects on lung permeability leakage and lung injury by upregulating Toll-interacting protein expression in rats with sepsis.

    Journal: International journal of molecular medicine

    doi: 10.3892/ijmm.2014.1943

    Figure 1. Effect of XBJ on the mRNA expression of Tollip, IRAK1, TLR4, NF-κB65 and TRAF6 in lung tissue. Groups of mice were challenged with CLP and treated with XBJ 24 h later. The expression of Tollip, IRAK1, TLR4, NF-κB65 and TRAF6 in lung tissue was determined by RT-PCR. Representative RT-PCR shows the level of Tollip, IRAK1, TLR4, NF-κB65, and TRAF6 expression in the four rat groups. M, marker; A, normal control group; B, sham operation group; C, control group; D, treatment group.
    Figure Legend Snippet: Figure 1. Effect of XBJ on the mRNA expression of Tollip, IRAK1, TLR4, NF-κB65 and TRAF6 in lung tissue. Groups of mice were challenged with CLP and treated with XBJ 24 h later. The expression of Tollip, IRAK1, TLR4, NF-κB65 and TRAF6 in lung tissue was determined by RT-PCR. Representative RT-PCR shows the level of Tollip, IRAK1, TLR4, NF-κB65, and TRAF6 expression in the four rat groups. M, marker; A, normal control group; B, sham operation group; C, control group; D, treatment group.

    Techniques Used: Expressing, Reverse Transcription Polymerase Chain Reaction, Marker, Control

    Figure 2. Administration of XBJ led to increased expression levels of Tollip mRNA, and inhibition of TLR4, NF-κB65 and TRAF6 mRNA expression in lung tissue in CLP-ALI mice. The expression of Tollip, IRAK1, TLR4, NF-κB65 and TRAF6 in lung tissue was determined by RT-PCR. Statistical summary of the densitometric analysis of Tollip, IRAK1, TLR4, NF-κB65 and TRAF6 expression in the four rat groups. Data are presented as mean ± stan dard deviation of one experiment consisting of three replicates. Experiments were performed in triplicate; ﹡P<0.05 and ﹡﹡P<0.01 vs. normal control group and sham operation group. ﹟P<0.05 and ﹟﹟P<0.01 vs. control group.
    Figure Legend Snippet: Figure 2. Administration of XBJ led to increased expression levels of Tollip mRNA, and inhibition of TLR4, NF-κB65 and TRAF6 mRNA expression in lung tissue in CLP-ALI mice. The expression of Tollip, IRAK1, TLR4, NF-κB65 and TRAF6 in lung tissue was determined by RT-PCR. Statistical summary of the densitometric analysis of Tollip, IRAK1, TLR4, NF-κB65 and TRAF6 expression in the four rat groups. Data are presented as mean ± stan dard deviation of one experiment consisting of three replicates. Experiments were performed in triplicate; ﹡P<0.05 and ﹡﹡P<0.01 vs. normal control group and sham operation group. ﹟P<0.05 and ﹟﹟P<0.01 vs. control group.

    Techniques Used: Expressing, Inhibition, Reverse Transcription Polymerase Chain Reaction, Control

    Figure 4. Administration of XBJ enhanced the expression of Tollip protein protein, and inhibition TLR4, NF-κB65, p-IRAK1 and TRAF6 protein expression in lung tissue in CLP-ALI mice. Groups of mice were challenged with LPS and treated with salidroside 24 h later. Tollip, p-IRAK1, TLR4, NF-κB65 and TRAF6 were assayed by western blot analysis. Statistical summary of the densitometric analysis of Tollip, p-IRAK1, TLR4, NF-κB65 and TRAF6 protein expression in the four rat groups. Data are presented as mean ± standard deviation of one experiment consisting of three replicates. Experiments were performed in triplicate; ﹡﹡P<0.01 vs. the normal control group and sham operation group. ﹟P<0.05, ﹟﹟P<0.01 vs. the control group.
    Figure Legend Snippet: Figure 4. Administration of XBJ enhanced the expression of Tollip protein protein, and inhibition TLR4, NF-κB65, p-IRAK1 and TRAF6 protein expression in lung tissue in CLP-ALI mice. Groups of mice were challenged with LPS and treated with salidroside 24 h later. Tollip, p-IRAK1, TLR4, NF-κB65 and TRAF6 were assayed by western blot analysis. Statistical summary of the densitometric analysis of Tollip, p-IRAK1, TLR4, NF-κB65 and TRAF6 protein expression in the four rat groups. Data are presented as mean ± standard deviation of one experiment consisting of three replicates. Experiments were performed in triplicate; ﹡﹡P<0.01 vs. the normal control group and sham operation group. ﹟P<0.05, ﹟﹟P<0.01 vs. the control group.

    Techniques Used: Expressing, Inhibition, Western Blot, Standard Deviation, Control

    Figure 3. Administration of XBJ enhanced the expression of Tollip protein, and inhibition of TLR4, NF-κB65, p-IRAK1 and TRAF6 protein expression in lung tissue in CLP-ALI mice. Groups of mice were challenged with LPS and treated with salidroside 24 h later. Tollip, p-IRAK1, TLR4, NF-κB65 and TRAF6 were assayed by western blot analysis. Representative western blots show the level of Tollip, p-IRAK1, TLR4, NF-κB65 and TRAF6 protein expression in the four rat groups. A, normal control group; B, sham operation group; C, control group; D, treatment group.
    Figure Legend Snippet: Figure 3. Administration of XBJ enhanced the expression of Tollip protein, and inhibition of TLR4, NF-κB65, p-IRAK1 and TRAF6 protein expression in lung tissue in CLP-ALI mice. Groups of mice were challenged with LPS and treated with salidroside 24 h later. Tollip, p-IRAK1, TLR4, NF-κB65 and TRAF6 were assayed by western blot analysis. Representative western blots show the level of Tollip, p-IRAK1, TLR4, NF-κB65 and TRAF6 protein expression in the four rat groups. A, normal control group; B, sham operation group; C, control group; D, treatment group.

    Techniques Used: Expressing, Inhibition, Western Blot, Control

    Figure 5. Administration of XBJ upregulated Tollip positive protein in lung tissue in CLP-ALI mice. Groups of mice were challenged with CLP and treated with XBJ 24 h later. Tollip positive protein levels of lung tissue were determined using immunohistochemistry and the average proportion of positive expres sion in each field was counted using the true color multi-functional cell image analysis management system. Values are expressed as mean ± SD; ﹡﹡P<0.01 vs. normal control group and sham operation group. ﹟P<0.05 and ﹟﹟P<0.01 vs. control group.
    Figure Legend Snippet: Figure 5. Administration of XBJ upregulated Tollip positive protein in lung tissue in CLP-ALI mice. Groups of mice were challenged with CLP and treated with XBJ 24 h later. Tollip positive protein levels of lung tissue were determined using immunohistochemistry and the average proportion of positive expres sion in each field was counted using the true color multi-functional cell image analysis management system. Values are expressed as mean ± SD; ﹡﹡P<0.01 vs. normal control group and sham operation group. ﹟P<0.05 and ﹟﹟P<0.01 vs. control group.

    Techniques Used: Immunohistochemistry, Functional Assay, Control

    Related Articles

    Expressing:

    Article Title: Xuebijing exerts protective effects on lung permeability leakage and lung injury by upregulating Toll-interacting protein expression in rats with sepsis.
    Article Snippet: GAPDH was obtained from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA).GAPDH was obtained from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA).. Rabbit anti-mouse Tollip, TLR4, TRAF6, p-IRAK1, VEGF-α, HO-1 and NF-κB polyclonal antibodies were purchased from Wuhan Boster Biological Technology, Ltd. (Wuhan, China).. Rabbit antimouse IRAK1 and phosphorylated IRAK1 were purchased from Cell Signaling, Technology (Beverly, MA, USA).Rabbit antimouse IRAK1 and phosphorylated IRAK1 were purchased from Cell Signaling, Technology (Beverly, MA, USA).

    Article Title: Xuebijing exerts protective effects on lung permeability leakage and lung injury by upregulating Toll-interacting protein expression in rats with sepsis
    Article Snippet: GAPDH was obtained from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA).GAPDH was obtained from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA).. Rabbit anti-mouse Tollip, TLR4, TRAF6, p-IRAK1, VEGF-α, HO-1 and NF-κB polyclonal antibodies were purchased from Wuhan Boster Biological Technology, Ltd. (Wuhan, China).. Rabbit anti-mouse IRAK1 and phosphorylated IRAK1 were purchased from Cell Signaling, Technology (Beverly, MA, USA).Rabbit anti-mouse IRAK1 and phosphorylated IRAK1 were purchased from Cell Signaling, Technology (Beverly, MA, USA).

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Xuebijing exerts protective effects on lung permeability leakage and lung injury by upregulating Toll-interacting protein expression in rats with sepsis.
    Article Snippet: GAPDH was obtained from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA).GAPDH was obtained from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA).. Rabbit anti-mouse Tollip, TLR4, TRAF6, p-IRAK1, VEGF-α, HO-1 and NF-κB polyclonal antibodies were purchased from Wuhan Boster Biological Technology, Ltd. (Wuhan, China).. Rabbit antimouse IRAK1 and phosphorylated IRAK1 were purchased from Cell Signaling, Technology (Beverly, MA, USA).Rabbit antimouse IRAK1 and phosphorylated IRAK1 were purchased from Cell Signaling, Technology (Beverly, MA, USA).

    Article Title: Xuebijing exerts protective effects on lung permeability leakage and lung injury by upregulating Toll-interacting protein expression in rats with sepsis
    Article Snippet: GAPDH was obtained from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA).GAPDH was obtained from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA).. Rabbit anti-mouse Tollip, TLR4, TRAF6, p-IRAK1, VEGF-α, HO-1 and NF-κB polyclonal antibodies were purchased from Wuhan Boster Biological Technology, Ltd. (Wuhan, China).. Rabbit anti-mouse IRAK1 and phosphorylated IRAK1 were purchased from Cell Signaling, Technology (Beverly, MA, USA).Rabbit anti-mouse IRAK1 and phosphorylated IRAK1 were purchased from Cell Signaling, Technology (Beverly, MA, USA).

    Marker:

    Article Title: Xuebijing exerts protective effects on lung permeability leakage and lung injury by upregulating Toll-interacting protein expression in rats with sepsis.
    Article Snippet: GAPDH was obtained from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA).GAPDH was obtained from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA).. Rabbit anti-mouse Tollip, TLR4, TRAF6, p-IRAK1, VEGF-α, HO-1 and NF-κB polyclonal antibodies were purchased from Wuhan Boster Biological Technology, Ltd. (Wuhan, China).. Rabbit antimouse IRAK1 and phosphorylated IRAK1 were purchased from Cell Signaling, Technology (Beverly, MA, USA).Rabbit antimouse IRAK1 and phosphorylated IRAK1 were purchased from Cell Signaling, Technology (Beverly, MA, USA).

    Article Title: Xuebijing exerts protective effects on lung permeability leakage and lung injury by upregulating Toll-interacting protein expression in rats with sepsis
    Article Snippet: GAPDH was obtained from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA).GAPDH was obtained from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA).. Rabbit anti-mouse Tollip, TLR4, TRAF6, p-IRAK1, VEGF-α, HO-1 and NF-κB polyclonal antibodies were purchased from Wuhan Boster Biological Technology, Ltd. (Wuhan, China).. Rabbit anti-mouse IRAK1 and phosphorylated IRAK1 were purchased from Cell Signaling, Technology (Beverly, MA, USA).Rabbit anti-mouse IRAK1 and phosphorylated IRAK1 were purchased from Cell Signaling, Technology (Beverly, MA, USA).

    Control:

    Article Title: Xuebijing exerts protective effects on lung permeability leakage and lung injury by upregulating Toll-interacting protein expression in rats with sepsis.
    Article Snippet: GAPDH was obtained from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA).GAPDH was obtained from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA).. Rabbit anti-mouse Tollip, TLR4, TRAF6, p-IRAK1, VEGF-α, HO-1 and NF-κB polyclonal antibodies were purchased from Wuhan Boster Biological Technology, Ltd. (Wuhan, China).. Rabbit antimouse IRAK1 and phosphorylated IRAK1 were purchased from Cell Signaling, Technology (Beverly, MA, USA).Rabbit antimouse IRAK1 and phosphorylated IRAK1 were purchased from Cell Signaling, Technology (Beverly, MA, USA).

    Article Title: Xuebijing exerts protective effects on lung permeability leakage and lung injury by upregulating Toll-interacting protein expression in rats with sepsis
    Article Snippet: GAPDH was obtained from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA).GAPDH was obtained from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA).. Rabbit anti-mouse Tollip, TLR4, TRAF6, p-IRAK1, VEGF-α, HO-1 and NF-κB polyclonal antibodies were purchased from Wuhan Boster Biological Technology, Ltd. (Wuhan, China).. Rabbit anti-mouse IRAK1 and phosphorylated IRAK1 were purchased from Cell Signaling, Technology (Beverly, MA, USA).Rabbit anti-mouse IRAK1 and phosphorylated IRAK1 were purchased from Cell Signaling, Technology (Beverly, MA, USA).

    Inhibition:

    Article Title: Xuebijing exerts protective effects on lung permeability leakage and lung injury by upregulating Toll-interacting protein expression in rats with sepsis.
    Article Snippet: GAPDH was obtained from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA).GAPDH was obtained from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA).. Rabbit anti-mouse Tollip, TLR4, TRAF6, p-IRAK1, VEGF-α, HO-1 and NF-κB polyclonal antibodies were purchased from Wuhan Boster Biological Technology, Ltd. (Wuhan, China).. Rabbit antimouse IRAK1 and phosphorylated IRAK1 were purchased from Cell Signaling, Technology (Beverly, MA, USA).Rabbit antimouse IRAK1 and phosphorylated IRAK1 were purchased from Cell Signaling, Technology (Beverly, MA, USA).

    Article Title: Xuebijing exerts protective effects on lung permeability leakage and lung injury by upregulating Toll-interacting protein expression in rats with sepsis
    Article Snippet: GAPDH was obtained from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA).GAPDH was obtained from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA).. Rabbit anti-mouse Tollip, TLR4, TRAF6, p-IRAK1, VEGF-α, HO-1 and NF-κB polyclonal antibodies were purchased from Wuhan Boster Biological Technology, Ltd. (Wuhan, China).. Rabbit anti-mouse IRAK1 and phosphorylated IRAK1 were purchased from Cell Signaling, Technology (Beverly, MA, USA).Rabbit anti-mouse IRAK1 and phosphorylated IRAK1 were purchased from Cell Signaling, Technology (Beverly, MA, USA).

    Western Blot:

    Article Title: Xuebijing exerts protective effects on lung permeability leakage and lung injury by upregulating Toll-interacting protein expression in rats with sepsis.
    Article Snippet: GAPDH was obtained from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA).GAPDH was obtained from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA).. Rabbit anti-mouse Tollip, TLR4, TRAF6, p-IRAK1, VEGF-α, HO-1 and NF-κB polyclonal antibodies were purchased from Wuhan Boster Biological Technology, Ltd. (Wuhan, China).. Rabbit antimouse IRAK1 and phosphorylated IRAK1 were purchased from Cell Signaling, Technology (Beverly, MA, USA).Rabbit antimouse IRAK1 and phosphorylated IRAK1 were purchased from Cell Signaling, Technology (Beverly, MA, USA).

    Article Title: Xuebijing exerts protective effects on lung permeability leakage and lung injury by upregulating Toll-interacting protein expression in rats with sepsis
    Article Snippet: GAPDH was obtained from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA).GAPDH was obtained from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA).. Rabbit anti-mouse Tollip, TLR4, TRAF6, p-IRAK1, VEGF-α, HO-1 and NF-κB polyclonal antibodies were purchased from Wuhan Boster Biological Technology, Ltd. (Wuhan, China).. Rabbit anti-mouse IRAK1 and phosphorylated IRAK1 were purchased from Cell Signaling, Technology (Beverly, MA, USA).Rabbit anti-mouse IRAK1 and phosphorylated IRAK1 were purchased from Cell Signaling, Technology (Beverly, MA, USA).

    Standard Deviation:

    Article Title: Xuebijing exerts protective effects on lung permeability leakage and lung injury by upregulating Toll-interacting protein expression in rats with sepsis.
    Article Snippet: GAPDH was obtained from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA).GAPDH was obtained from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA).. Rabbit anti-mouse Tollip, TLR4, TRAF6, p-IRAK1, VEGF-α, HO-1 and NF-κB polyclonal antibodies were purchased from Wuhan Boster Biological Technology, Ltd. (Wuhan, China).. Rabbit antimouse IRAK1 and phosphorylated IRAK1 were purchased from Cell Signaling, Technology (Beverly, MA, USA).Rabbit antimouse IRAK1 and phosphorylated IRAK1 were purchased from Cell Signaling, Technology (Beverly, MA, USA).

    Article Title: Xuebijing exerts protective effects on lung permeability leakage and lung injury by upregulating Toll-interacting protein expression in rats with sepsis
    Article Snippet: GAPDH was obtained from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA).GAPDH was obtained from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA).. Rabbit anti-mouse Tollip, TLR4, TRAF6, p-IRAK1, VEGF-α, HO-1 and NF-κB polyclonal antibodies were purchased from Wuhan Boster Biological Technology, Ltd. (Wuhan, China).. Rabbit anti-mouse IRAK1 and phosphorylated IRAK1 were purchased from Cell Signaling, Technology (Beverly, MA, USA).Rabbit anti-mouse IRAK1 and phosphorylated IRAK1 were purchased from Cell Signaling, Technology (Beverly, MA, USA).

    Immunohistochemistry:

    Article Title: Xuebijing exerts protective effects on lung permeability leakage and lung injury by upregulating Toll-interacting protein expression in rats with sepsis.
    Article Snippet: GAPDH was obtained from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA).GAPDH was obtained from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA).. Rabbit anti-mouse Tollip, TLR4, TRAF6, p-IRAK1, VEGF-α, HO-1 and NF-κB polyclonal antibodies were purchased from Wuhan Boster Biological Technology, Ltd. (Wuhan, China).. Rabbit antimouse IRAK1 and phosphorylated IRAK1 were purchased from Cell Signaling, Technology (Beverly, MA, USA).Rabbit antimouse IRAK1 and phosphorylated IRAK1 were purchased from Cell Signaling, Technology (Beverly, MA, USA).

    Article Title: Xuebijing exerts protective effects on lung permeability leakage and lung injury by upregulating Toll-interacting protein expression in rats with sepsis
    Article Snippet: GAPDH was obtained from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA).GAPDH was obtained from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA).. Rabbit anti-mouse Tollip, TLR4, TRAF6, p-IRAK1, VEGF-α, HO-1 and NF-κB polyclonal antibodies were purchased from Wuhan Boster Biological Technology, Ltd. (Wuhan, China).. Rabbit anti-mouse IRAK1 and phosphorylated IRAK1 were purchased from Cell Signaling, Technology (Beverly, MA, USA).Rabbit anti-mouse IRAK1 and phosphorylated IRAK1 were purchased from Cell Signaling, Technology (Beverly, MA, USA).

    Functional Assay:

    Article Title: Xuebijing exerts protective effects on lung permeability leakage and lung injury by upregulating Toll-interacting protein expression in rats with sepsis.
    Article Snippet: GAPDH was obtained from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA).GAPDH was obtained from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA).. Rabbit anti-mouse Tollip, TLR4, TRAF6, p-IRAK1, VEGF-α, HO-1 and NF-κB polyclonal antibodies were purchased from Wuhan Boster Biological Technology, Ltd. (Wuhan, China).. Rabbit antimouse IRAK1 and phosphorylated IRAK1 were purchased from Cell Signaling, Technology (Beverly, MA, USA).Rabbit antimouse IRAK1 and phosphorylated IRAK1 were purchased from Cell Signaling, Technology (Beverly, MA, USA).

    Article Title: Xuebijing exerts protective effects on lung permeability leakage and lung injury by upregulating Toll-interacting protein expression in rats with sepsis
    Article Snippet: GAPDH was obtained from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA).GAPDH was obtained from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA).. Rabbit anti-mouse Tollip, TLR4, TRAF6, p-IRAK1, VEGF-α, HO-1 and NF-κB polyclonal antibodies were purchased from Wuhan Boster Biological Technology, Ltd. (Wuhan, China).. Rabbit anti-mouse IRAK1 and phosphorylated IRAK1 were purchased from Cell Signaling, Technology (Beverly, MA, USA).Rabbit anti-mouse IRAK1 and phosphorylated IRAK1 were purchased from Cell Signaling, Technology (Beverly, MA, USA).

    Microarray:

    Article Title: Xuebijing exerts protective effects on lung permeability leakage and lung injury by upregulating Toll-interacting protein expression in rats with sepsis.
    Article Snippet: GAPDH was obtained from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA).GAPDH was obtained from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA).. Rabbit anti-mouse Tollip, TLR4, TRAF6, p-IRAK1, VEGF-α, HO-1 and NF-κB polyclonal antibodies were purchased from Wuhan Boster Biological Technology, Ltd. (Wuhan, China).. Rabbit antimouse IRAK1 and phosphorylated IRAK1 were purchased from Cell Signaling, Technology (Beverly, MA, USA).Rabbit antimouse IRAK1 and phosphorylated IRAK1 were purchased from Cell Signaling, Technology (Beverly, MA, USA).

    Article Title: Xuebijing exerts protective effects on lung permeability leakage and lung injury by upregulating Toll-interacting protein expression in rats with sepsis
    Article Snippet: GAPDH was obtained from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA).GAPDH was obtained from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA).. Rabbit anti-mouse Tollip, TLR4, TRAF6, p-IRAK1, VEGF-α, HO-1 and NF-κB polyclonal antibodies were purchased from Wuhan Boster Biological Technology, Ltd. (Wuhan, China).. Rabbit anti-mouse IRAK1 and phosphorylated IRAK1 were purchased from Cell Signaling, Technology (Beverly, MA, USA).Rabbit anti-mouse IRAK1 and phosphorylated IRAK1 were purchased from Cell Signaling, Technology (Beverly, MA, USA).



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    Figure 1. Effect of XBJ on the mRNA expression of <t>Tollip,</t> IRAK1, TLR4, NF-κB65 and TRAF6 in lung tissue. Groups of mice were challenged with CLP and treated with XBJ 24 h later. The expression of Tollip, IRAK1, TLR4, NF-κB65 and TRAF6 in lung tissue was determined by RT-PCR. Representative RT-PCR shows the level of Tollip, IRAK1, TLR4, NF-κB65, and TRAF6 expression in the four rat groups. M, marker; A, normal control group; B, sham operation group; C, control group; D, treatment group.
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    Image Search Results


    Figure 1. Effect of XBJ on the mRNA expression of Tollip, IRAK1, TLR4, NF-κB65 and TRAF6 in lung tissue. Groups of mice were challenged with CLP and treated with XBJ 24 h later. The expression of Tollip, IRAK1, TLR4, NF-κB65 and TRAF6 in lung tissue was determined by RT-PCR. Representative RT-PCR shows the level of Tollip, IRAK1, TLR4, NF-κB65, and TRAF6 expression in the four rat groups. M, marker; A, normal control group; B, sham operation group; C, control group; D, treatment group.

    Journal: International journal of molecular medicine

    Article Title: Xuebijing exerts protective effects on lung permeability leakage and lung injury by upregulating Toll-interacting protein expression in rats with sepsis.

    doi: 10.3892/ijmm.2014.1943

    Figure Lengend Snippet: Figure 1. Effect of XBJ on the mRNA expression of Tollip, IRAK1, TLR4, NF-κB65 and TRAF6 in lung tissue. Groups of mice were challenged with CLP and treated with XBJ 24 h later. The expression of Tollip, IRAK1, TLR4, NF-κB65 and TRAF6 in lung tissue was determined by RT-PCR. Representative RT-PCR shows the level of Tollip, IRAK1, TLR4, NF-κB65, and TRAF6 expression in the four rat groups. M, marker; A, normal control group; B, sham operation group; C, control group; D, treatment group.

    Article Snippet: Rabbit anti-mouse Tollip, TLR4, TRAF6, p-IRAK1, VEGF-α, HO-1 and NF-κB polyclonal antibodies were purchased from Wuhan Boster Biological Technology, Ltd. (Wuhan, China).

    Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Marker, Control

    Figure 2. Administration of XBJ led to increased expression levels of Tollip mRNA, and inhibition of TLR4, NF-κB65 and TRAF6 mRNA expression in lung tissue in CLP-ALI mice. The expression of Tollip, IRAK1, TLR4, NF-κB65 and TRAF6 in lung tissue was determined by RT-PCR. Statistical summary of the densitometric analysis of Tollip, IRAK1, TLR4, NF-κB65 and TRAF6 expression in the four rat groups. Data are presented as mean ± stan dard deviation of one experiment consisting of three replicates. Experiments were performed in triplicate; ﹡P<0.05 and ﹡﹡P<0.01 vs. normal control group and sham operation group. ﹟P<0.05 and ﹟﹟P<0.01 vs. control group.

    Journal: International journal of molecular medicine

    Article Title: Xuebijing exerts protective effects on lung permeability leakage and lung injury by upregulating Toll-interacting protein expression in rats with sepsis.

    doi: 10.3892/ijmm.2014.1943

    Figure Lengend Snippet: Figure 2. Administration of XBJ led to increased expression levels of Tollip mRNA, and inhibition of TLR4, NF-κB65 and TRAF6 mRNA expression in lung tissue in CLP-ALI mice. The expression of Tollip, IRAK1, TLR4, NF-κB65 and TRAF6 in lung tissue was determined by RT-PCR. Statistical summary of the densitometric analysis of Tollip, IRAK1, TLR4, NF-κB65 and TRAF6 expression in the four rat groups. Data are presented as mean ± stan dard deviation of one experiment consisting of three replicates. Experiments were performed in triplicate; ﹡P<0.05 and ﹡﹡P<0.01 vs. normal control group and sham operation group. ﹟P<0.05 and ﹟﹟P<0.01 vs. control group.

    Article Snippet: Rabbit anti-mouse Tollip, TLR4, TRAF6, p-IRAK1, VEGF-α, HO-1 and NF-κB polyclonal antibodies were purchased from Wuhan Boster Biological Technology, Ltd. (Wuhan, China).

    Techniques: Expressing, Inhibition, Reverse Transcription Polymerase Chain Reaction, Control

    Figure 4. Administration of XBJ enhanced the expression of Tollip protein protein, and inhibition TLR4, NF-κB65, p-IRAK1 and TRAF6 protein expression in lung tissue in CLP-ALI mice. Groups of mice were challenged with LPS and treated with salidroside 24 h later. Tollip, p-IRAK1, TLR4, NF-κB65 and TRAF6 were assayed by western blot analysis. Statistical summary of the densitometric analysis of Tollip, p-IRAK1, TLR4, NF-κB65 and TRAF6 protein expression in the four rat groups. Data are presented as mean ± standard deviation of one experiment consisting of three replicates. Experiments were performed in triplicate; ﹡﹡P<0.01 vs. the normal control group and sham operation group. ﹟P<0.05, ﹟﹟P<0.01 vs. the control group.

    Journal: International journal of molecular medicine

    Article Title: Xuebijing exerts protective effects on lung permeability leakage and lung injury by upregulating Toll-interacting protein expression in rats with sepsis.

    doi: 10.3892/ijmm.2014.1943

    Figure Lengend Snippet: Figure 4. Administration of XBJ enhanced the expression of Tollip protein protein, and inhibition TLR4, NF-κB65, p-IRAK1 and TRAF6 protein expression in lung tissue in CLP-ALI mice. Groups of mice were challenged with LPS and treated with salidroside 24 h later. Tollip, p-IRAK1, TLR4, NF-κB65 and TRAF6 were assayed by western blot analysis. Statistical summary of the densitometric analysis of Tollip, p-IRAK1, TLR4, NF-κB65 and TRAF6 protein expression in the four rat groups. Data are presented as mean ± standard deviation of one experiment consisting of three replicates. Experiments were performed in triplicate; ﹡﹡P<0.01 vs. the normal control group and sham operation group. ﹟P<0.05, ﹟﹟P<0.01 vs. the control group.

    Article Snippet: Rabbit anti-mouse Tollip, TLR4, TRAF6, p-IRAK1, VEGF-α, HO-1 and NF-κB polyclonal antibodies were purchased from Wuhan Boster Biological Technology, Ltd. (Wuhan, China).

    Techniques: Expressing, Inhibition, Western Blot, Standard Deviation, Control

    Figure 3. Administration of XBJ enhanced the expression of Tollip protein, and inhibition of TLR4, NF-κB65, p-IRAK1 and TRAF6 protein expression in lung tissue in CLP-ALI mice. Groups of mice were challenged with LPS and treated with salidroside 24 h later. Tollip, p-IRAK1, TLR4, NF-κB65 and TRAF6 were assayed by western blot analysis. Representative western blots show the level of Tollip, p-IRAK1, TLR4, NF-κB65 and TRAF6 protein expression in the four rat groups. A, normal control group; B, sham operation group; C, control group; D, treatment group.

    Journal: International journal of molecular medicine

    Article Title: Xuebijing exerts protective effects on lung permeability leakage and lung injury by upregulating Toll-interacting protein expression in rats with sepsis.

    doi: 10.3892/ijmm.2014.1943

    Figure Lengend Snippet: Figure 3. Administration of XBJ enhanced the expression of Tollip protein, and inhibition of TLR4, NF-κB65, p-IRAK1 and TRAF6 protein expression in lung tissue in CLP-ALI mice. Groups of mice were challenged with LPS and treated with salidroside 24 h later. Tollip, p-IRAK1, TLR4, NF-κB65 and TRAF6 were assayed by western blot analysis. Representative western blots show the level of Tollip, p-IRAK1, TLR4, NF-κB65 and TRAF6 protein expression in the four rat groups. A, normal control group; B, sham operation group; C, control group; D, treatment group.

    Article Snippet: Rabbit anti-mouse Tollip, TLR4, TRAF6, p-IRAK1, VEGF-α, HO-1 and NF-κB polyclonal antibodies were purchased from Wuhan Boster Biological Technology, Ltd. (Wuhan, China).

    Techniques: Expressing, Inhibition, Western Blot, Control

    Figure 5. Administration of XBJ upregulated Tollip positive protein in lung tissue in CLP-ALI mice. Groups of mice were challenged with CLP and treated with XBJ 24 h later. Tollip positive protein levels of lung tissue were determined using immunohistochemistry and the average proportion of positive expres sion in each field was counted using the true color multi-functional cell image analysis management system. Values are expressed as mean ± SD; ﹡﹡P<0.01 vs. normal control group and sham operation group. ﹟P<0.05 and ﹟﹟P<0.01 vs. control group.

    Journal: International journal of molecular medicine

    Article Title: Xuebijing exerts protective effects on lung permeability leakage and lung injury by upregulating Toll-interacting protein expression in rats with sepsis.

    doi: 10.3892/ijmm.2014.1943

    Figure Lengend Snippet: Figure 5. Administration of XBJ upregulated Tollip positive protein in lung tissue in CLP-ALI mice. Groups of mice were challenged with CLP and treated with XBJ 24 h later. Tollip positive protein levels of lung tissue were determined using immunohistochemistry and the average proportion of positive expres sion in each field was counted using the true color multi-functional cell image analysis management system. Values are expressed as mean ± SD; ﹡﹡P<0.01 vs. normal control group and sham operation group. ﹟P<0.05 and ﹟﹟P<0.01 vs. control group.

    Article Snippet: Rabbit anti-mouse Tollip, TLR4, TRAF6, p-IRAK1, VEGF-α, HO-1 and NF-κB polyclonal antibodies were purchased from Wuhan Boster Biological Technology, Ltd. (Wuhan, China).

    Techniques: Immunohistochemistry, Functional Assay, Control

    FIGURE 1. TOLLIP interrupts time-dependent expression of TLRs on M. A, cells were cultured with (black line) or without (shaded) HlyA and analyzed by flow cytometry for the expression of TLR1, -2, -4, and -6 at time points shown between 2–48 h. B, cell lysate was prepared after 10 h of incubation of M with andwithoutHlyA,electrophoresed,andimmunoblottedfordetectionofTOLLIP.Theblotwasreprobedwithanti--actinAbtoensureequalloadingofprotein in both lanes. C, M of C57BL/6 mice were cultured in absence and presence of HlyA or with anti-TLR4 Ab plus HlyA and analyzed for expression of TLR2 and -6. Up-regulation of TLR2 was also analyzed on M of C3H/HeJ mice treated with or without HlyA. The data given were obtained in one of three representative experiments. *, p 0.05.

    Journal: Journal of Biological Chemistry

    Article Title: Hemolysin Induces Toll-like Receptor (TLR)-independent Apoptosis and Multiple TLR-associated Parallel Activation of Macrophages

    doi: 10.1074/jbc.m111.241851

    Figure Lengend Snippet: FIGURE 1. TOLLIP interrupts time-dependent expression of TLRs on M. A, cells were cultured with (black line) or without (shaded) HlyA and analyzed by flow cytometry for the expression of TLR1, -2, -4, and -6 at time points shown between 2–48 h. B, cell lysate was prepared after 10 h of incubation of M with andwithoutHlyA,electrophoresed,andimmunoblottedfordetectionofTOLLIP.Theblotwasreprobedwithanti--actinAbtoensureequalloadingofprotein in both lanes. C, M of C57BL/6 mice were cultured in absence and presence of HlyA or with anti-TLR4 Ab plus HlyA and analyzed for expression of TLR2 and -6. Up-regulation of TLR2 was also analyzed on M of C3H/HeJ mice treated with or without HlyA. The data given were obtained in one of three representative experiments. *, p 0.05.

    Article Snippet: The cell lysates were electrophoresed, Western-blotted, and incubated overnight with rabbit antimouse TOLLIP mAb (Santa Cruz Biotechnology), anti-mouse Bcl-xL, caspase-6, or caspase-7 Ab (Cell Signaling Technology).

    Techniques: Expressing, Cell Culture, Flow Cytometry, Incubation